Understanding how the structure and function of the microbiome impacts health and disease. In particular I am interested in the intersection of bioinformatics, gnotobiotics (germ-free laboratory animals), and microbiology to dissect complex bacterial communities.
We use Petunia inflata as a model to study biochemical, molecular, and structural bases of a self/non-self recognition mechanism between pollen and pistil adopted by flowering plants to prevent in breeding and promote outcrossing.
The research in my lab is mainly focused on the mechanism of the disease Friedreich’s Ataxia, associated with expansion of GAA trinucleotide. Additionally, my research is attempting to determine the mechanism of repeats expansion during their transmission from generation to generation.
The combination of tools from functional genomics, molecular biology, computational biology, biochemistry, and metabolomics to understand the fundamental molecular mechanisms underlying the development of this parasite.
We use microbial genetics, biochemistry, and cell biology approaches to determine the molecular mechanisms that enable bacteria to establish symbiosis with a eukaryotic host. The model system is the symbiosis formed between the bioluminescent bacterium Vibrio fischeri and the Hawaiian bobtail squid Euprymna scolopes. Our primary interests in this system include quorum sensing,contact-dependent killing mechanisms, and sulfur metabolism.
Our lab is the birthplace of Galaxy. It is developed and maintained together with our collaborators and worldwide community. We also work on a variety of topics related to mutational dynamics and experimental evolution. We are also an integral part of the AnVIL project.
Understanding the host-metabolite-microbiota communication network‚ specifically how the manipulation of gut microbiota by diet and/or xenobiotics impacts host metabolites (e.g., bile acids, short chain fatty acids), their metabolism, and how these co-metabolites interact with host ligand-activated transcription factors.
Stress-induced gene expression and UV resistance pathways, Regulation of mRNAs from birth to death during stress responses, Targeted protein degradation during transcriptional stress and How RNA Polymerase II contends with barriers throughout the genome.
The Rolls lab aims to understand how neurons generate axons and dendrites with different microtubule organization, and how neurons respond to injury. Current projects focus on mechanisms that control microtubule polarity and dynamics and mechanisms that promote neuronal regeneration.
We study the signal transduction and vesicular trafficking processes that promote migration in epithelial cells. We seek to understand the role of these processes in normal homeostasis and in pathological processes.
Neurovirology, Genomics of Pathogen Variation, Neuron-Virus Relationships
Understanding the consequences of HSV latency for the neurons that harbor the HSV pathogen and the search for improved therapeutics using a combination of virology, neurobiology, next generation sequencing technologies, and bioinformatics.
Elucidating molecular structures relevant to chemists, biochemists, material scientists etc. and educating graduate students embarking in these fields, the technique of X-ray diffraction (crystal growth, data collection and structure solution and refinement, and interpretation).