Tim Miyashiro

About Me

Dr. Miyashiro joined the Department of Biochemistry and Molecular Biology Department at Penn State in 2013.  His laboratory studies the squid-vibrio symbiosis to increase understanding of the mechanisms by which bacteria establish symbiosis with animals.  He trained as a postdoctoral fellow in Dr. Edward G. Ruby’s laboratory at University of Wisconsin-Madison.  He earned his Ph.D. in Physics in Dr. Mark Goulian’s laboratory at the University of Pennsylvania.  He earned his B.S. in Physics at the University of Delaware.

Department or University Committees

• Graduate Program Steering Committee
   
• Graduate Student Recruiting Committee
   
• Microbiome Center Executive Committee

Program or Departmental Affiliations

Centers

Research Interest

Understanding of the mechanisms by which bacteria establish symbiosis

Research Summary

All animals, including humans, have evolved in a microbial world, and their physiology depends on intimate associations, i.e., symbioses, with bacteria.  We call these bacteria 'symbionts', and remarkably, many of these symbionts are acquired from the environment.  The Miyashiro lab is seeking to increase understanding of the mechanisms by which bacteria establish symbiosis.  We use approaches based on microbiology, genetics, biochemistry, and cell biology to explore these mechanisms in the context of a natural animal-microbe symbiosis comprised of a Hawaiian squid (Euprymna scolopes) and a bioluminescent bacterium (Vibrio fischeri).

Teaching and Mentoring

Microbes have had profound effects on Earth's history and the evolution of higher organisms, including humans.  I teach three microbiology courses:

1. MICRB 201: Introductory Microbiology
    
2. MICRB 203: Introductory Microbiology Lab (Inquiry-based)
    
3. BMB 488-006: Community of Practice in Biochemistry and Molecular Biology: Host-Microbe Interactions

Honors and Awards

Selected Publications

List of Peer-Reviewed Publications (28 total; ^† = undergraduate student):

Guckes KR, Yount TA^†, Steingard CH^†, and Miyashiro TI. (2023) Quorum sensing inhibits interference competition among bacterial symbionts within a host. Curr Biol 33:4244-4251.

Provencher EAP, Ehrig MR^†, Cecere AG, Cousins SC, Maybin MA, Meredith TC, and Miyashiro TI. (2023) Inhibition of biofilm formation by a lipopolysaccharide-associated glycosyltransferase in the bacterial symbiont Vibrio fischeri. Frontiers in Bacteriology 2:1254305.

Cecere AG, Cook RA^†, and Miyashiro TI. (2023) A case study assessing the impact of mating frequency on the reproductive performance of the Hawaiian bobtail squid Euprymna scolopes. Laboratory Animal Research 39:17.

Donnelly AR^†, Giacobe EJ^†, Cook RA^†, Francis GM^†, Buddle GK^†, Beaubrun CL^†, Cecere AG, and Miyashiro TI. (2023) Quantification of the capacity of Vibrio fischeri to establish symbiosis with Euprymna scolopes. PLoS One 18:e0287519.

Surrett ED, Guckes KR, Cousins S^†, Ruskoski TB, Cecere AG, Ludvik DA, Okafor CD, Mandel MJ, Miyashiro TI. (2023) Two enhancer binding proteins activate σ54-dependent transcription of a quorum regulatory RNA in a bacterial symbiont. Elife 12:e78544.

Guckes KR and Miyashiro TI. (2023) The type-VI secretion system of the beneficial symbiont Vibrio fischeri. Microbiology (Reading). 169:001302.

Yount TA^†, Murtha AN^†, Cecere AG, and Miyashiro TI. Quorum sensing facilitates interpopulation signaling by Vibrio fischeri within the light organ of Euprymna scolopes. Israel Journal of Chemistry 63:e20220062.

Cecere AG and Miyashiro TI. (2022) Impact of transit time on the reproductive capacity of Euprymna scolopes as a laboratory animal.  Laboratory Animal Research 38:25.

Wasilko NP, Ceron JS, Baker ER^†, Cecere AG, Wollenberg MS, and Miyashiro T. (2021) Vibrio fischeri imports and assimilates sulfate during symbiosis with Euprymna scolopes. Molecular Microbiology 116:926-42.

• Guckes KR, Cecere AG, Wasilko NP, Williams AL^†, Bultman KM, Mandel MJ, and Miyashiro T. (2019) Incompatibility of Vibrio fischeri strains during symbiosis establishment depends on two hcp genes.  Journal of Bacteriology 201:e00221-19.
   
• Bultman KM, Cecere AG, Miyashiro T, Septer AN, and Mandel MJ. (2019) Draft genome sequences of type VI secretion system-encoding Vibrio fischeri strains FQ-A001 and ES401.  Microbiology Resource Announcements 8:e00385-19.
   
• Wasilko NP, Larios-Valencia J^†, Steingard CH^†, Nunez B^†, Verma SC, and Miyashiro T. (2018) Sulfur availability for Vibrio fischeri growth during symbiosis establishment depends on biogeography within the squid light organ. Molecular Microbiology 111:621-36.
   
• Speare L, Cecere AG, Guckes KR, Wollenberg M, Mandel MJ, Miyashiro T*, and Septer AN. (2018) Bacterial symbionts use a type VI secretion system to eliminate competitors in their natural host.  Proceedings of the National Academies of Sciences of the United States of America 115:E8528-37.
  * = co-corresponding author
   
• Verma SC and Miyashiro T. (2016) Niche-specific impact of a symbiotic function on the persistence of microbial symbionts within a natural host.  Applied and Environmental Microbiology 82:5990-6.
   
• Sun Y, LaSota ED^†, Cecere AG, LaPenna KB^†, Larios-Valencia J^†, Wollenberg MS, and Miyashiro T. (2016) Intraspecific competition impacts Vibrio fischeri strain diversity during initial colonization of the squid light organ.  Applied and Environmental Microbiology 82:3082-91.
   
• Sun Y, Verma SC, Bogale H^†, and Miyashiro T. (2015) NagC represses N-acetyl-glucosamine utilization genes in Vibrio fischeri within the light organ of Euprymna scolopes.  Frontiers in Microbiology 6:741.
   
• Miyashiro T, Oehlert D^†, Ray VA, Visick KL, and Ruby EG. (2014) The putative oligosaccharide translocase SypK connects biofilm formation with quorum signaling in Vibrio fischeri. MicrobiologyOpen 3:836-48.
   
• Koch EJ, Miyashiro T, McFall-Ngai MJ, and Ruby EG. (2014) Features governing symbiont persistence in the squid-vibrio association. Molecular Ecology 23:1624-34.
   
• Verma SC and Miyashiro T. (2013) Quorum sensing in the squid-Vibrio symbiosis. International Journal of Molecular Sciences 14:16386-401.
   
• Sun Y, Bernardy EE, Hammer BK, and Miyashiro T. (2013) Competence and natural transformation in vibrios. Molecular Microbiology 89: 583-95.
   
• Miyashiro T and Ruby EG. (2012). Shedding light on bioluminescence regulation in Vibrio fischeri. Molecular Microbiology 84: 795-806.
   
• Cao X, Studer S, Wasserman KM, Zhang Y, Ruby EG, and Miyashiro T. (2012) The novel sigma factor-like regulator RpoQ controls luminescence, chitinase activity and motility in Vibrio fischeri. mBio 3: e00285-11.
   
• Miyashiro T, Klein W^†, Oehlert D^†, Cao X, Schwartzman J, and Ruby EG. (2011) The N-acetyl-D-glucosamine repressor NagC of Vibrio fischeri facilitates colonization of Euprymna scolopes. Molecular Microbiology 82: 894-903.
   
• Miyashiro T, Wollenberg MS, Cao X, Oehlert D^†, and Ruby EG. (2010) A single qrr gene is necessary and sufficient for LuxO-mediated regulation in Vibrio fischeri. Molecular Microbiology 77: 1556-67.
   
• Miyashiro T and Goulian M. (2008) High stimulus unmasks positive feedback in an autoregulated bacterial signaling circuit. Proceedings of the National Academies of Sciences of the United States of America 105: 17457-62.
   
• Liu Z, Miyashiro T, Tsou A, Hsiao A, Goulian M, and Zhu J. (2008) Mucosal penetration primes Vibrio cholerae for host colonization by repressing quorum sensing. Proceedings of the National Academies of Sciences of the United States of America 105: 9769-74.
   
• Miyashiro T and Goulian M. (2007) Stimulus-dependent differential regulation in the Escherichia coli PhoQ-PhoP system. Proceedings of the National Academies of Sciences of the United States of America 104: 16305-10.