Shardul Kulkarni

About Me

I joined as a graduate student in the lab of Dr. Vasudevan Seshadri at NCCS, India. After receiving my Ph.D. in Biotechnology from University of Pune, India in 2013, I worked as a visiting fellow in the lab of Dr. Jon Lorsch at National Institutes of Health, USA. I am currently working as a Research Assistant Professor at BMMB in the lab of Dr. Joseph Reese at Pennsylvania State University. 

Program or Departmental Affiliations

• The BMMB Graduate Program

Editorial Boards

• Editor, Publons

Centers

• The Center for Eukaryotic Gene Regulation (CEGR)

Research Interest

Gene expression regulation in eukaryotic cells

Research Summary

My research focus has been on understanding the gene regulatory mechanisms in eukaryotic cells. In my Ph.D. I worked on elucidating the mechanism of glucose stimulated translational regulation of insulin mRNA. As a post-doc at NIH, I worked on genome-wide analysis of upstream translation in yeast cells. I specifically focused on understanding the stress-dependent changes in the accuracy of translation regulation.

Currently, I am working on deciphering the genome-wide and proteome-wide functional significance of CCR4-NOT complex using CRISPR.

Honors and Awards

• Ph.D. (Biotechnology) 2013
   
• M.Sc. (Zoology) 2007
   
• B.Sc. (Microbiology) 2005 

Selected Publications

• Glucose-stimulated translation regulation of insulin by the 5′ UTR-binding proteins SD Kulkarni, B Muralidharan, AC Panda, B Bakthavachalu, A Vindu, ... Journal of Biological Chemistry 286 (16), 14146-14156 (PMID: 21357685).
   
• miR-196b-mediated translation regulation of mouse insulin2 via the 5′ UTR AC Panda, I Sahu, SD Kulkarni, JL Martindale, K Abdelmohsen, A Vindu, ... PloS one 9 (7), e101084 (PMID: 25003985).
   
• eIF1A residues implicated in cancer stabilize translation preinitiation complexes and favor suboptimal initiation sites in yeast P Martin-Marcos, F Zhou, C Karunasiri, F Zhang, J Dong, J Nanda, ... Elife 6, e31250 (PMID: 29206102).
   
• Novel splice variant of mouse insulin2 mRNA: implications for insulin expression AC Panda, SD Kulkarni, B Muralidharan, B Bakthavachalu, V Seshadri FEBS letters 584 (6), 1169-1173 (PMID: 20153322).
   
• Temperature-dependent regulation of upstream open reading frame translation in S. cerevisiae SD Kulkarni, F Zhou, ND Sen, H Zhang, AG Hinnebusch, JR Lorsch BMC biology 17 (1), 1-27 (PMID: 31810458).
   
• eIF1 discriminates against suboptimal initiation sites to prevent excessive uORF translation genome-wide F Zhou, H Zhang, SD Kulkarni, JR Lorsch, AG Hinnebusch RNA 26 (4), 419-438 (PMID: 31915290).
   
• Translation of insulin granule proteins are regulated by PDI and PABP RD Sarwade, A Khalique, SD Kulkarni, PR Pandey, N Gaikwad, ... Biochemical and Biophysical Research Communications (PMID: 32248978).