I grew up on a diary farm in north central Kentucky and became interested in science at an early age. I fulfilled a long-time dream by attending Massachusetts Institute of Technology as an undergraduate, where I obtained a B. Sc. with honors in Chemistry and Biology in 1972. I became interested in bioenergetics as an undergraduate, so I moved to University of California-Los Angeles for graduate school, where I became a member of the first class of students in a new NIH-sponsored training program in molecular biology. I quickly decided that photosynthesis was the topic I wished to pursue in the fall of 1972, and I started working with Alexander N. Glazer and Frederick Eiserling, beginning a lifelong research affiliation with cyanobacteria. After completing my Ph. D. training, I was a postdoctoral fellow in the laboratory of Roger Y. Stanier and Germaine Cohen-Bazire at the Institut Pasteur in Paris from 1977 to 1979, and I then joined the laboratory of Roderick K. Clayton at Cornell University from 1979 to 1981. Finally, I joined the faculty at Penn State in September 1981 and have been here since then, rising to the level of professor in 1991. I spent sabbaticals in Switzerland (1989-1990), Australia (1996-1997), Rockville, MD (TIGR), and Bozeman, Montana (twice; 2010-2011 and 2017-2018).
I am now a microbial (eco)physiologist, who has y applied cutting-edge genomics, genetics, biochemistry, and molecular biology to study chlorophototrophic bacteria—Cyanobacteria, Chloroflexi, Chlorobi, and Acidobacteria—for 47 years. My recent studies demonstrating that cyanobacteria extensively remodel their photosynthetic apparatus by synthesizing new chlorophylls, phycobiliproteins, and reaction centers in far-red light explains how these organisms can grow at the bottom of dense microbial mats [Science 345:1312-17 (2014) and Science 353:aaf9178 (2016)]. Introduction of this capability into crop plants could significantly increase their productivity by expanding the usable light for photosynthesis into the far-red range (700–800 nm). In an elegant example of my broad expertise to solve a previously intractable problem, we elucidated (bacterio-)chlorophyll and carotenoid biosynthetic pathways in green sulfur bacteria, and this directly led to structures for the BChl supramolecular structures in chlorosomes, their light-harvesting complexes. My deep knowledge of photosynthesis and metabolism is represented by studies of microbial mats in Yellowstone hot springs, where 17 chlorophototrophic members from six phyla and ~325 total bacteria “species” occur. Using systems biology/-omics approaches, we and our collaborators have made impressive progress in advancing our understanding of the structure, interdependence, and integration of members of such communities. The discovery of Chloracidobacterium thermophilum [Science 317:523-526 (2007)], the first chlorophototrophic member of the phylum Acidobacteria, is already featured in microbiology textbooks. Finally, our recent demonstration that cyanobacteria have a complete tricarboxylic acid cycle decisively overturned the belief, accepted for ~50 years, that the cyanobacterial TCA cycle is branched [Science 334:1551-1553 (2011)] and provides new opportunities for metabolic engineering. These are just a few highlights of a research career spanning five decades. To date, I have published (or submitted) ~420 papers and books since 1972, including about 100 papers since 2014. These research products have received ~25,338 total citations (>8,568 since 2014). The h-index for these publications is 82 (82 publications with ≥82 citations; 47 since 2014) and the i10-index is 326 (i.e., 326 papers with ≥10 citations) and 220 with 10 new citations since 2014. The book “The Molecular Biology of Cyanobacteria,” published in 1994, has received about 4,000 total citations.
|Massachusetts Institute of Technology||Chemistry/Biology||B. Sc.||1972|
|University of California, Los Angeles||Molecular Biology||Ph. D.||1977|
|Institut Pasteur (RY Stanier, G Cohen-Bazire)||Microbiology||Postdoc||1977-1979|
|Cornell University (RK Clayton)||Biophysics||Postdoc||1979-1981|
|1981—present||Assistant, Associate, (1986) and Full Professor (1991), Department of Biochemistry and Molecular Biology, The Pennsylvania State University|
|2009—2015||Adjunct Research Professor, Chemistry & Biochemistry, Montana State University|
|2013—2018||Visiting Professor, Singapore Centre for Environmental Life Sciences Engineering, Nanyang Technological University, Singapore|
|2015— present||Research Professor, Chemistry & Biochemistry, Montana State University|
Genomics, structural and functional relationships, metabolism, physiology and ecology of chlorophototrophic bacteria
Genomics, structural and functional relationships, metabolism, physiology and ecology of chlorophototrophic bacteria
Photosynthesis, the chlorophyll-dependent conversion of light energy into chemical energy with the ensuing reduction of carbon dioxide to biomass, is the most important biological process on Earth. Among prokaryotes, the ability to use chlorophylls to capture and convert light into biochemical energy was until very recently believed to occur in members of only five eubacterial kingdoms: Cyanobacteria, Proteobacteria, Chlorobi, Chloroflexi, and Firmicutes. We recently discovered a previously unknown chlorophototroph, Chloracidobacterium thermophilum, which is a member of the poorly characterized kingdom Acidobacteria (http://www.sciencemag.org/cgi/content/abstract/317/5837/523). Current research in my laboratory focuses on a wide variety of topics in photosynthesis in bacteria, including structure-function relationships of proteins, biogenesis of the photosynthetic apparatus, gene regulation, and photosynthetic physiology. We have principally studied two model organisms, the unicellular, marine cyanobacterium Synechococcus sp. PCC 7002 and the moderately thermophilic green sulfur Chlorobaculum tepidum (Chlorobi), which can easily be manipulated genetically. More recently, we have studied several terretrial cyanobacteria that can acclimate to use far-red/niear-infra red radiation to perform oxygenic photosynthesis. Because Cyanobacteria perform oxygen-evolving photosynthesis but characterized Chlorobi are obligately photoautotrophic anaerobes, these two organisms provide an interesting contrast in physiology and metabolism. Finally, we additionally study natural chlorophototrophic communities in mat communities in Yellowstone National Park, WY.
Long-term objectives of my laboratory are to understand the structure, function, assembly, and regulation of expression of bacterial photosynthetic apparatuses, principally those of members of the phyla Cyanobacteria and Chlorobi. To achieve these goals, we have obtained complete genomic sequences for all of the organisms we study. These data enable bioinformatics approaches that facilitate gene discovery and characterization. In total, genome sequences for about 100 chlorophototroph organisms have been determined since 2000. We additionally use nextGen sequencing methods for transcription profiling of Synechococcus sp. PCC 7002 and Cba. tepidum, as well as for profiling gene expression patterns of the entire mat community from which Cab. thermophilum was isolated (see Figure 1). In a recent “Multi-omics Analysis Experiment” (MOAE, the “Mother Of All Experiments”), >15,000 genes were tracked in samples collected at hourly intervals over one diurnal cycle.
Figure 1. Left, Octopus Spring, an alkaline siliceous hot sprin in the Lower Geyser Basin of Yellowstone National Park, WY. Right, microbial mat showing the upper, 1-2 mm green chlorophototrophic community from which Candidatus Chloracidobacterium thermophilum was isolated. The lower red layers are carotenoid-containing members of the anoxic community.
The photosynthetic apparatus of cyanobacteria closely resembles that found in the chloroplasts of higher plants. We have developed sophisticated genetic tools to analyze gene function in Synechococcus sp. PCC 7002, and these have been used for metabolic engineering to improve biosolar hydrogen, biomass, and biofuels production in this robust cyanobacterium. The Chlorobi are specifically adapted for survival in low-light environments and are important in reducing carbon and nitrogen while oxidizing sulfide in anoxic environments. We have developed a reliable natural transformation method for Cba. tepidum and have used this capability to define the pathways for bacteriochlorophyll and carotenoid biosynthesis in this organism as well as to characterize the structure of chlorophylls in the light-harvesting organelle, the chlorosome (Figure 2).
Figure 2. Single and double-layer model of bacteriochlorophyll d structure in chlorosomes of a bchQRU mutant of Chlorobaculum tepidum; see Ganapathy et al. (2009) Proc. Natl. Acad. Sci. USA 106: 8515-8530 for details).
Students and postdoctoral associates apply a broad combination of methods including microbial ecology, microbial physiology, genomics and bioinformatics, molecular genetics, protein biochemistry, and spectroscopic methods. We collaborate extensively with Dr. John H. Golbeck (http://www.bmb.psu.edu/faculty/golbeck/golbeck.html), Dr. David M. Ward of Montana State University (see http://landresources.montana.edu/dward/), among numerous other scientists world-wide.
Honors and Awards
B. Sc. in Chemistry with honors, Massachusetts Institute of Technology
|1972 - 1976||
U. S. Public Health Service Pre-doctoral Traineeship, Univ. of California-Los Angeles (Advisors: Alexander N. Glazer and Frederick A. Eiserling)
|1977 - 1979||
N.S.F.-C.N.R.S. Postdoctoral fellowship (U.S.--France exchange program).
|1992 - present||
Ernest C. Pollard Professor of Biotechnology
|1995 - present||
Fellow, American Academy of Microbiology
Daniel R. Tershak Memorial Teaching Award, Dept. of Biochemistry and Molecular Biology, The Pennsylvania State University
Brown & Williamson Distinguished Lecturer, University of Louisville, Department of Biology
|2011 - present||
Fellow, American Association for the Advancement of Science
Daniel I. Arnon Lecturer, University of California, Berkeley
|2012 - 2018||
Member (elected), Board of Governors, American Academy of Microbiology
D. C. White Research and Mentoring Award, American Society for Microbiology
214. Bryant, D. A., Garcia Costas, A. M., Maresca, J. A., Gomez Maqueo Chew, A., Klatt, C. G., Bateson, M. M., Tallon, L. J. Hostetler, J., Nelson, W. C., Heidelberg, J. F., Ward, D. M. 2007. “Candidatus Chloracidobacterium thermophilum”: an aerobic phototrophic acidobacterium. Science 317, 523–526.
278. Zhang, S. and Bryant, D. A. 2011. The cyanobacterial tricarboxylic acid cycle. Science 334, 1551–1553. 278.
329. Gan, F., Zhang, S., Rockwell, N. C., Martin, S. S., Lagarias, J. C. and Bryant, D. A. 2014. Extensive remodeling of a cyanobacterial photosynthetic apparatus in far-red light. Science 345, 1312–1317.
370. Ho, M.-Y., Shen, G., Canniffe, D. P., Zhao, C., and Bryant, D. A. 2016. Light-dependent chlorophyll f synthase is a highly divergent paralog of PsbA of Photosystem II. Science 353, aaf9178. doi: 10.1126/science.aaf9178.
393. Martin, W. F., Bryant, D. A. and Beatty, J. T. 2018. A physiological perspective on the origin and evolution of photosynthesis. FEMS Microbiol. Rev. 42, 201–231. doi: 10.1093/femsre/fux056
394. Bryant, D. A., and Canniffe, D. P. 2018. How Nature designs antenna proteins: design principles and functional realization of light-harvesting antenna systems in chlorophototrophic prokaryotes. J. Phys. B: At. Mol. Opt. Phys. 51, 033001. doi: 10.1088/1361-6455/aa9c3c
395. Chen, G. E., Canniffe, D. P, Barnett, S. F. H., Hollingshead, S., Brindley A. A., Vasilev, C., Bryant, D. A. Hunter, C. N. 2018. Complete enzyme set for chlorophyll biosynthesis in Escherichia coli. Sci. Adv. 4, eaaq1407. doi: 10.1126/sciadv.aaq1407.
396. Thiel, V., Tank, M. and Bryant, D. A. 2018. Diversity of chlorophototrophic bacteria revealed in the omics era. Annu. Rev. Plant Biol. 69, 21–49. doi: 10.1146/annurev-arplant-042817-040500
397. Bryant, D. A. 2018. Phototrophy and phototrophs. In: Encyclopedia of Microbiology, 4th edition, Schmidt, T. editor-in-chief. Elsevier, Amsterdam, pp. 527–537.
398. Mancini, J. A., Sheehan, M., Kodali, G., Chow, B. Y., Bryant, D. A., Dutton, P. L., and Moser, C. C. 2018. De novo synthetic biliprotein design, assembly and excitation energy transfer. J. R. Soc. Interface 20180021. doi: 10.1098/rsif.2018.0021
399. Ortega-Ramos, M., Canniffe, D. P., Radle, M. I., Hunter, C. N., Bryant, D. A., Golbeck, J. H. 2018. Engineered biosynthesis of bacteriochlorophyll gF in Rhodobacter sphaeroides. Biochim. Biophys. Acta 1859, 501–509. doi: 10.1016/j.bbabio.2018.02.006
400. Zill, J. C., He, Z., Tank, M., Canniffe, D. P., Ferlez, B. H., Lahav, Y., Bellstedt, P., Alia, A., Schapiro, I., Golbeck, J. H., Bryant, D. A. and Matysik, J. 2018. 15N-Photo-CIDNP MAS NMR analysis of reaction centers of Chloracidobacterium thermophilum. Photosynth. Res. 137, 295–305. doi: 10.1007/s11120-018-0504-1
401. Blain-Hartung, M., Rockwell, N. C., Moreno, M. V., Martin, S. S., Gan, F., Bryant, D. A., and Lagarias, J. C. 2018. Cyanobacteriochrome-based photoswitchable adenylyl cyclases (cPACs) for broad spectrum light regulation of cAMP levels in cells. J. Biol. Chem. 293, 8473–8483. doi: 10.1074/jbc.RA118.002258
402. Günther, L. M., Löhner, A., Reiher, C., Kunsel, T., Jansen, T. L. C., Tank, M., Bryant, D. A., Knoester, J., and Köhler, J. 2018. Structural variations in chlorosomes from wild-type and a bchQR mutant of Chlorobaculum tepidum revealed by single-molecule spectroscopy. J. Phys. Chem. Part B, 122, 6712–6723. doi: 10.1021/acs.jpcb.8b02875
403. Cherepanov, D. A., Milanovsky, G. E., Gopta, O. A., Balasubramanian, R., Bryant, D. A., Semenov, A. Yu., and Golbeck, J. H. 2018. Electron-phonon coupling in cyanobacterial photosystem I. J. Phys. Chem. Part B, 122, 7943–7955. doi: 10.1021/acs.jpcb.8b03906
404. Canniffe, D. P., Thweatt, J. L., Gomez Maqueo Chew, A., Hunter, C. N. and Bryant, D. A. 2018. A paralog of phytol reductase catalyzes the formation of 1,2-dihydro-carotenoids in green sulfur bacteria. J. Biol. Chem. 293, 15233–15242. doi:10.1074/jbc.RA118.004672
405. Tank, M., Garcia Costas, A. M., and Bryant, D. A. 2018. Genus: Chloracidobacterium. In: Bergey’s Manual of Systematics of Bacteria and Archaea (W. B. Whitman, supervising editor), John Wiley & Sons, New York. doi: 10.1002/9781118960608.
406. Swainsbury, D. K., Faries, K. M., Niedzwiedzki, D. M., Martin, E. C., Finders, A. J., Canniffe, D. P., Shen, G., Bryant, D. A., Kirmaier, C., Holten, D. and Hunter, C. N. 2019. Engineering of B800 bacteriochlorphyll binding site specificity in Rhodobacter sphaeroides LH2. Biochim. Biophys. Acta 1860, 209-223. doi: 10.1016/j.bbabio.2018.11.008
407. Thiel, V., Garcia Costas, A. M., Fortney, N. W. W., Martinez, J. N., Roden, E. E., Boyd, E. S., Ward, D. M. and Bryant, D. A. 2019. “Candidatus Thermonerobacter thiotrophicus,” a non-phototrophic, sulfate-reducing member of the phylum Chlorobi that inhabits hot-spring communities. Front. Microbiol. 9, 3159. doi: 10.3389/fmicb.2018.03159
408. Shen, G., Canniffe, D. P., Kurashov, V., Ho, M.-Y., van der Est, A., Golbeck, J. H. and Bryant, D. A. 2019. Improved heterologous expression of chlorophyll f synthase in Synechococcus sp. PCC 7002: isolation and initial characterization. Photosynth. Res. 140, 77-92. doi: 10.1007/s11120-018-00610-9
409. Kurashov, V., Ho, M.-Y., Shen, G., Piedl, K., Laremore, T. N., Bryant, D. A. and Golbeck, J. H. 2019. Energy transfer from chlorophyll f to the trapping center in naturally occurring and engineered Photosystem I complexes. Photosynth. Res. 141, 151-163. doi: 10.1007/s11120-019-006. 16-x
410. Kumaraswamy, G. K., Krishnan, A., Ananyev, G., Zhang, S., Bryant, D. A., Dismukes, G. C. 2019. Crossing the Thauer limit: rewiring cyanobacterial metabolism to maximize fermentative H2 production. Energy Environ. Sci., 12, 1035–1045. doi: 10.1039/c8ee03606c.
411. Ho, M.-Y., Bryant, D. A. 2019. Global transcription profiling of the cyanobacterium Chlorogloeopsis fritschii PCC 9212 in far-red light: insights into the regulation of chlorophyll d biosynthesis. Front. Microbiol. 10, 465. doi: 10.3389/fmicb.2019.00465
412. Thweatt, J. L., Canniffe, D. P., and Bryant, D. A. 2019. Biosynthesis of chlorophylls and bacteriochlorophylls in green bacteria. Grimm, B., ed., Advances in Botanical Research, Vol. 90, pp. 35-89. Metabolism, Structure and Function of Chlorophylls, B. Grimm, ed. Elsevier, Amsterdam. Invited review, doi: 10.1016/bs.abr.2019.03.002
413. Hastings, G., Makita, H., Agarwala, N., Rohani, L., Shen, G. and Bryant, D. A. 2019. Fourier-transform visible and infrared difference spectroscopy for the study of P700 in photosystem I of Fischerella thermalis PCC 7521 cells grown under white light and far-red light: evidence that cofactor A–1 is chlorophyll f. Biochim. Biophys. Acta 1860, 452–460. doi: 10.1016/j.bbabio.2019.04.002
414. He, Z., Kurashov, V., Ferlez, B., Tank, M., Golbeck, J. H., and Bryant, D. A. 2019. Homodimeric type-1 reaction centers of Chloracidobacterium thermophilum (Acidobacteria): I. Biochemical and biophysical characterization. Photosynth. Res. 142, 87-103. doi: 10.1007/s11120-019-00650-9
415. Ho, M.-Y., Niedzwiedzki, D. M., MacGregor-Chatwin C., Gerstenecker, G., Hunter, C. N., Blankenship, R. E., and Bryant, D. A. 2019. Extensive remodeling of the photosynthetic apparatus alters energy transfer among photosynthetic complexes when cyanobacteria acclimate to far-red light. Biochim. Biophys. Acta—Bioenergetics, invited article, in press. doi: 10.1016/j.bbabio.2019.148064
416. Gisriel, C., Shen, G., Kurashov, V., Ho, M.-Y., Zhang, S., Williams, D., Golbeck, J. H., Fromme, P., Bryant, D. A. 2019. The structure of Photosystem I acclimated to far-red light illuminates an ecologically important acclimation process in photosynthesis. Sci. Adv., revision resubmitted.
417. Bryant, D. A., Shen, G., Turner, G. M., Soulier, N., Laremore, T. N., and Ho, M.-Y. 2019. Far-red-light allophycocyanin subunits play a role in chlorophyll d accumulation. Photosynth. Res., submitted for publication.
418. Cherepanov, D. A., Shelaev, I. V., Gostev, F. E., Aybush, A. V., Mamedov, M. D., Shen, G., Nadtochenko, V. A., Bryant, D. A., Semenov, A. Yu., and Golbeck, J. H. 2019. Chlorophyll f functions exclusively in photon capture and energy transfer in far-red-light photosystem I from Fischerella thermalis PCC 7521. Biochim. Biophys. Acta—Bioenergetics, submitted for publication.
419. Steinke, L., Slysz, G. W., Klatt, C. G., Moran, J. J., Lipton, M. S., Romine, M. F., Woods, J. Anderson, G., Bryant, D. A. and Ward, D. M. 2019. Short-term stable isotope probing of proteins reveals taxa incorporating inorganic carbon in a hot spring microbial mat. Appl. Environ. Microbiol., submitted for publication.
420. Charles, P., Kalendra, V., He, Z, Khatima, M., Golbeck, J. H., van der Est, A., Lakshmi, K. V., and Bryant, D. A. 2019. Zn-Bacteriochlorophyll a is the primary donor (P840) in the Type-1 reaction centers of Chloracidobacterium thermophilum. J. Am. Chem. Soc., in preparation.