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CANCELLED: CAMP Seminar: Bulk light-scattering measurements of viral capsid self-assembly around RNA.
Add to Calendar 2020-04-20T19:30:00 2020-04-20T20:30:00 UTC CANCELLED: CAMP Seminar: Bulk light-scattering measurements of viral capsid self-assembly around RNA.

Physics CAMP Seminar

Davey Lab (339)
Start DateMon, Apr 20, 2020
3:30 PM
to
End DateMon, Apr 20, 2020
4:30 PM
Presented By
LaNell Williams, Harvard

Physics CAMP Seminar

Self-assembly is a vital part of the of the RNA virus life cycle. The assembly of viral coat proteins around viral RNA occurs both in vivo and in vitro, suggesting that viral capsid assembly may be driven by a minimization of free energy. To better understand this process, we modify the interactions between coat proteins and between the coat proteins and RNA of MS2 bacteriophage in vitro by varying the ionic strength and pH, and we study the assembly using dynamic and static light scattering. From dynamic light scattering we determine the assembly yield and the size distribution of assembled products. From static light scattering, we measure the kinetics of assembly in bulk. By comparing the results from these two different techniques to each other and to results from gel electrophoresis, we infer features of the assembly pathway.